I have made my way to Ambon since the last blog, where I have been preparing the last logistics with my local colleagues from Pattimura University before the actual fieldwork begins. In the last 3 days, the other team members have also started arriving, with the final team member (and master fish counter) landing tomorrow morning. The main things that had to happen before our boat leaves port (besides recovering from jetlag), was organising a detailed plan, training new team members, and preparing all the gear.
One of the things we will be doing, is collecting environmental DNA (eDNA) to study biodiversity on coral reefs. If you want to know more about eDNA, I have written more about it here or here. In short: eDNA are tiny fragments of DNA in the water column that come from poop, mucus, etc. By filtering and analysing a scoop of water, we can tell what lives in the area we took water from. Because eDNA is such a new method, most people have not used it before. So in a great mutual benefit arrangement, our Pattimura University colleagues took us (my colleague Dom and me) out for a dive and in return we showed them how to collect water 😉
We obviously did more than just collecting seawater, we also went back to the lab to teach them the protocols on how to filter samples while avoiding contamination. Since eDNA analysis is so good at picking up the tiniest fragments of DNA, a careless brush of a fingertip can render the entire sample useless. We are collecting data from a boat instead of a high-tech lab, so being aware of how things can go wrong is absolutely crucial to get reliable data.
Tomorrow morning we set sail (start engine?) for 10 days of research around Ambon. So today we had make sure all the equipment got to the boat, for us to leave at first light in the morning. Between dive gear (including compressors, tanks, etc), survey tools, eDNA equipment, and other random practical bits and pieces, it took multiple returns trips with the pickup to get everything to the boat. Science is of course hungry work and feeding 16 people takes a lot of grocery shopping, which was luckily taken care of by our local team. Gino (our Ambon trip leader) has assured me we have an excellent chef on board, so be aware that there is a decent chance that the rest of these fieldwork blogs will mostly be about tasty Indonesian food!
As we will be on a boat for quite a while in the next weeks, I am not sure yet if I will be able to post blogs until we are back on the mainland. If I can snatch up some 4G signal along the way, you’ll be able to read an update on the fieldwork in a couple of days. Otherwise, the next blog will be online around the 11th of October.